Tris, commonly used as a buffer in biochemistry and molecular biology, stabilizes the pH of solutions in electrophoresis and other biological applications. It is also an enzyme inhibitor used in the bio-pharmaceutical industry and is commonly utilized in electro chromatography, UV analysis and HPLC applications. Tris is extensively used as a biological buffer or a component of buffer solutions.Triscanbeused as a “Good’s” buffer because it has low UV absorptivity, minimal reactivity, stable pH and is soluble in water.
Characters | Unit | Specification |
---|---|---|
Absorbance (40%) | a.u | 0.2 a.u. max. |
Appearance and Color | White / Crystals | |
Assay | % | 99.0–101.0% |
Bacterial Endotoxins | EU/gram | 3.0 EU/gram max. |
Enzymes: | ||
DNase | None Detected | |
RNase | None Detected | |
Protease | None Detected | |
Heavy Metals | ppm | 5 ppm max. |
Identification B | Passes Test | |
Identification C | Passes Test | |
Identification (IR) | Passes Test | |
Insoluble Matter | % | 0.005% max. |
Karl Fischer Water | % | 2% max. |
LOD | % | 1.0 % max. |
Melting Range | oC | 168 – 172 oC |
Microbial Content: | ||
TAMC | CFU/g max | 1000 CFU/g max. |
TYMC | CFU/g max | 100 CFU/g max. |
pH (1 in 20) | 10.0 – 11.5 | |
Residue on Ignition | % | 0.1% max. |
Trace Metals: | ||
Arsenic (As) | ppm | 5 ppm max. |
Calcium (Ca) | ppm | 5 ppm max. |
Copper (Cu) | ppm | 5 ppm max. |
Iron (Fe) | ppm | 5 ppm max. |
Lead (Pb) | ppm | 5 ppm max. |
Shelf Life/Retest Date: The recommended retest period for Tromethamine is two years from the date of manufacture.
Storage/Shipping Conditions:
Pack Sizes: 1 Kg