Tromethamine (Tris)


CAS #:


Molecular Formula:




Molecular Weight:

121.14 g/mol


Tris, commonly used as a buffer in biochemistry and molecular biology, stabilizes the pH of solutions in electrophoresis and other biological applications. It is also an enzyme inhibitor used in the bio-pharmaceutical industry and is commonly utilized in electro chromatography, UV analysis and HPLC applications. Tris is extensively used as a biological buffer or a component of buffer solutions.Triscanbeused as a “Good’s” buffer because it has low UV absorptivity, minimal reactivity, stable pH and is soluble in water.

Characters Unit Specification
Absorbance (40%) a.u 0.2 a.u. max.
Appearance and Color White / Crystals
Assay % 99.0–101.0%
Bacterial Endotoxins EU/gram 3.0 EU/gram max.
DNase None Detected
RNase None Detected
Protease None Detected
Heavy Metals ppm 5 ppm max.
Identification B Passes Test
Identification C Passes Test
Identification (IR) Passes Test
Insoluble Matter % 0.005% max.
Karl Fischer Water % 2% max.
LOD % 1.0 % max.
Melting Range oC 168 – 172 oC
Microbial Content:
TAMC CFU/g max 1000 CFU/g max.
TYMC CFU/g max 100 CFU/g max.
pH (1 in 20) 10.0 – 11.5
Residue on Ignition % 0.1% max.
Trace Metals:
Arsenic (As) ppm 5 ppm max.
Calcium (Ca) ppm 5 ppm max.
Copper (Cu) ppm 5 ppm max.
Iron (Fe) ppm 5 ppm max.
Lead (Pb) ppm 5 ppm max.

Shelf Life/Retest Date: The recommended retest period for Tromethamine is two years from the date of manufacture.

Storage/Shipping Conditions:

  • Ship and store in ambient temperature.

Pack Sizes: 1 Kg

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