Tromethamine (Tris)
USP, BIO FUISA Grade
CAS #:
77-86-1
Molecular Formula:
NH2C(CH2OH)3
Synonym:
Tris
Molecular Weight:
121.14 g/mol
Overview
Tris, commonly used as a buffer in biochemistry and molecular biology, stabilizes the pH of solutions in electrophoresis and other biological applications. It is also an enzyme inhibitor used in the bio-pharmaceutical industry and is commonly utilized in electro chromatography, UV analysis and HPLC applications. Tris is extensively used as a biological buffer or a component of buffer solutions.Triscanbeused as a “Good’s” buffer because it has low UV absorptivity, minimal reactivity, stable pH and is soluble in water.
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| Characters | Unit | Specification |
|---|---|---|
| Absorbance (40%) | a.u | 0.2 a.u. max. |
| Appearance and Color | White / Crystals | |
| Assay | % | 99.0–101.0% |
| Bacterial Endotoxins | EU/gram | 3.0 EU/gram max. |
| Enzymes: | ||
| DNase | None Detected | |
| RNase | None Detected | |
| Protease | None Detected | |
| Heavy Metals | ppm | 5 ppm max. |
| Identification B | Passes Test | |
| Identification C | Passes Test | |
| Identification (IR) | Passes Test | |
| Insoluble Matter | % | 0.005% max. |
| Karl Fischer Water | % | 2% max. |
| LOD | % | 1.0 % max. |
| Melting Range | oC | 168 – 172 oC |
| Microbial Content: | ||
| TAMC | CFU/g max | 1000 CFU/g max. |
| TYMC | CFU/g max | 100 CFU/g max. |
| pH (1 in 20) | 10.0 – 11.5 | |
| Residue on Ignition | % | 0.1% max. |
| Trace Metals: | ||
| Arsenic (As) | ppm | 5 ppm max. |
| Calcium (Ca) | ppm | 5 ppm max. |
| Copper (Cu) | ppm | 5 ppm max. |
| Iron (Fe) | ppm | 5 ppm max. |
| Lead (Pb) | ppm | 5 ppm max. |
Shelf Life/Retest Date: The recommended retest period for Tromethamine is two years from the date of manufacture.
Storage/Shipping Conditions:
- Ship and store in ambient temperature.
Pack Sizes: 1 Kg
