Tris HCl

Bio Pharma Grade

CAS #:


Molecular Formula:

NH2C(CH2OH)3 • HCl


2-Amino-2-(Hydroxymethyl)-1,3-PropanediolHydrochloride, Tris (Hydroxymethyl) AminomethaneHydrochloride

Molecular Weight:

157.60 g/mol


Tris Hydrochloride is a stabilizing buffer in biological applications such as electrochromatography, UV analysis and HPLC. It is used to adjust and stabilize the pH ranges for gels used in electrophoresis applications. Tris Hydrochloride is extensively used as a biological buffer or a component of buffer solutions.

Characters Unit Specification
Absorbance (1M) 280 nm a. u. 0.06 a.u. max.
Appearance and colour White/Crystals
Assay (Dried) % 99.5 % min
Dnase None detected
Protease None detected
Rnase None detected
Heavy metals ppm 2 ppm max
Identification IR Passes test
Insoluble Matter % 0.001 % max
LOD at 105°C % 0.5 % max
Melting range °C 150- 153
pH (0.5M) 4.0- 5.0
pKa 8.0- 8.4
Residue on Ignition % 0.1 % max
Solubility (1M) Passes test
Trace Metals:
Arsenic (As) ppm 5 ppm max
Calcium (Ca) ppm 5 ppm max
Copper (Cu) ppm 5 ppm max
Iron (Fe) ppm 5 ppm max
Lead (Pb) ppm 5 ppm max
Magnesium (Mg) ppm 5 ppm max

Shelf Life/Retest Date: The recommended retest period for Tromethamine Hydrochloride is two years from the date of manufacture.

Storage/Shipping Conditions:

  • Store in a cool, dry, well-ventilated area away from incompatible substances.

Pack Sizes: 1 Kg

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