Tris HCl
Bio Pharma Grade
CAS #:
1185-53-1
Molecular Formula:
NH2C(CH2OH)3 • HCl
Synonym:
2-Amino-2-(Hydroxymethyl)-1,3-PropanediolHydrochloride, Tris (Hydroxymethyl) AminomethaneHydrochloride
Molecular Weight:
157.60 g/mol
Overview
Tris Hydrochloride is a stabilizing buffer in biological applications such as electrochromatography, UV analysis and HPLC. It is used to adjust and stabilize the pH ranges for gels used in electrophoresis applications. Tris Hydrochloride is extensively used as a biological buffer or a component of buffer solutions.
| Characters | Unit | Specification |
|---|---|---|
| Absorbance (1M) 280 nm | a. u. | 0.06 a.u. max. |
| Appearance and colour | White/Crystals | |
| Assay (Dried) | % | 99.5 % min |
| Enzymes: | ||
| Dnase | None detected | |
| Protease | None detected | |
| Rnase | None detected | |
| Heavy metals | ppm | 2 ppm max |
| Identification | IR | Passes test |
| Insoluble Matter | % | 0.001 % max |
| LOD at 105°C | % | 0.5 % max |
| Melting range | °C | 150- 153 |
| pH (0.5M) | 4.0- 5.0 | |
| pKa | 8.0- 8.4 | |
| Residue on Ignition | % | 0.1 % max |
| Solubility (1M) | Passes test | |
| Trace Metals: | ||
| Arsenic (As) | ppm | 5 ppm max |
| Calcium (Ca) | ppm | 5 ppm max |
| Copper (Cu) | ppm | 5 ppm max |
| Iron (Fe) | ppm | 5 ppm max |
| Lead (Pb) | ppm | 5 ppm max |
| Magnesium (Mg) | ppm | 5 ppm max |
Shelf Life/Retest Date: The recommended retest period for Tromethamine Hydrochloride is two years from the date of manufacture.
Storage/Shipping Conditions:
- Store in a cool, dry, well-ventilated area away from incompatible substances.
Pack Sizes: 1 Kg
