Guanidine Hydrochloride
Pharma Grade
CAS #:
50-01-1
Molecular Formula:
CH5N3•HCl
Synonym:
Carbamidine Hydrochloride, Iminourea Hydrochloride, Aminomethanamidine Hydrochloride
Molecular Weight:
95.53 g/mol
Overview
Guanidine Hydrochloride is a strong protein denaturant that functions as a chaotropic agent. As a denaturant, it acts to unfold proteins and turn them into their original polypeptide chains. As a chaotropic agent, it breaks down the structure of proteins. Guanidine Hydrochloride is commonly used in the purification of RNA by dissociating the RNA into its nucleic acids and protein forms. At higher concentrations, Guanidine Hydrochloride decreases enzyme activity. It is also used to increase the solubility of hydrophobic molecules.
| Characters | Unit | Specification |
|---|---|---|
| Absorbance: | ||
| 260 nm | a.u | 0.0300 a.u. maximum |
| 275 nm | a.u | 0.0300 a.u. maximum |
| 230 nm | a.u | 0.2000 a.u. maximum |
| Appearance and Color | White / Crystals | |
| Assay | % | 99.5% minimum |
| Enzymes: | ||
| DNase | None Detected | |
| Protease | None Detected | |
| RNase | None Detected | |
| Identification (IR) | Passes Test | |
| Insoluble Matter | % | 0.15% maximum |
| Loss on Drying | % | 0.5% maximum |
| Melting Range | oC | 184-188ºC |
| Nitrate | % | 0.01% maximum |
| pH (6M) | 4.5-6.0 | |
| Residue on Ignition | % | 0.05% maximum |
| Solubility (6M) | Passes Test | |
| Sulfate | % | 0.01% maximum |
| Trace Metals: | ||
| Arsenic (As) | ppm | 5 ppm maximum |
| Copper (Cu) | ppm | 5 ppm maximum |
| Iron (Fe) | ppm | 5 ppm maximum |
| Lead (Pb) | ppm | 5 ppm maximum |
Shelf Life/Retest Date: The recommended retest period for GuanidineHydrochloride, CAS number – 50-01-1 is 2 years from the date of manufacture.
Storage/Shipping Conditions:
- Ship and store in an ambient temperature.
- Keep containers tightly closed.
Pack Sizes:1 Kg
