Glycerol is used both in sample preparation and gel formation for polyacrylamide gel electrophoresis. Glycerol (5-10%) increases the density of a sample so that the sample will layer at the bottom of a gel’s sample well. Glycerol is also used to aid in casting gradient gels and as a protein stabilizer and storage buffer component.
Glycerol is odorless, colorless, viscous in nature, and exists as a sweet tasting liquid. It can be derived naturally as well as from petrochemical feedstock. Glycerol has a wide variety of applications, and is one of the most valuable and versatile chemical substances in nature. It can be used as an emollient, solvent, sweetening agent, in pharmaceutical formulations, injections, cosmetics, foodstuffs and toiletries. It is very stable and can be easily stored under normal temperature; also, it is non-irritating and has no adverse impact to the environment.
Characters | Unit | Specification |
---|---|---|
Appearance | colourless or almost colourless, clear liquid | |
Assay | % | 99.0 – 101.0 |
Identification (A, B, C) | complies | |
Appearance of solution | clear and colourless | |
Specific density | g/cm3 | min. 1.249 |
Acidity or alkalinity | ml of 0,1 M NaOH | max. 0.2 |
Residue on ignition / Sulphated ash | % | max. 0.01 |
Chloride | ppm | max. 10 |
Refractive index | 1.470 – 1.475 | |
Aldehydes | ppm | max. 10 |
Esters | ml of 0,1M HCl | min 8.0 |
Water | % | max. 2.0 |
Halogenated compounds | ppm | max. 35 |
Sugars | complies | |
Sulphate | ppm | max. 20 |
Heavy metals | ppm | max. 5 |
Total of all impurities | % | max. 0.5 |
Total aerobic microbial count | cfu/g | max. 100 |
Total yeasts and moulds count | cfu/g | max. 50 |
E.coli | cfu/g | absent in 1g |
Pseudomonas aeruginosa | cfu/g | absent in 1g |
Staphylococcus aureus | cfu/g | absent in 1g |
Salmonella | cfu/g | absent in 25g |
Bacterial endotoxine | IU/g | max. 10 |
Shelf Life/Retest Date: Glycerol 99% can be kept for at least 1 and half years if stored under recommended conditions.
Storage/Shipping Conditions:
Pack Sizes: 6 Kg